Journal: Biomedical Reports
Article Title: Ethyl acetate fraction of Curcuma longa leaves suppresses IL-6-induced STAT3 activation via ERK signaling in Hep3B cells
doi: 10.3892/br.2026.2108
Figure Lengend Snippet: CL-E inhibits IL-6-induced expression of CRP and SOCS3 mRNA in Hep3B cells. Hep3B cells were pretreated with CL-E at concentrations of 10, 30, and 60 µg/ml for 1 h, followed by IL-6 stimulation (10 ng/ml) for 6 h. Total RNA was extracted, and the mRNA levels of (A) CRP and (B) SOCS3 were measured using quantitative real-time PCR. Gene expression levels were normalized to 18S rRNA and are presented as fold change relative to the untreated control. Data represent the mean ± SE (n≥3). * P<0.05, ** P<0.01 and *** P<0.001 vs. the IL-6-treated group; ### P<0.001. CL-E, Curcuma longa L. extract; IL-6, interleukin 6; CRP, C reactive protein; P-, phosphorylated; SOCS3, suppressor of cytokine signaling 3.
Article Snippet: TaqMan primers for human CRP (Hs04183452_g1) and SOCS3 (Hs02330328_s1) were obtained from Applied Biosystems.
Techniques: Expressing, Real-time Polymerase Chain Reaction, Gene Expression, Control